Effect of perfluorononanoic acid (PFNA) on early embryo development in vitro
For the last decades a concern has been raised that female fertility is declining – more than could be explained by the fact that we choose to have children later in life and possible genetic...
In vivo modeling of high grade glioma for oncology drug developments
The main goal of this study was to develop a platform for quantifying the tumor-initiating capacity of a large panel of glioma-stem cell cultures (GSCs) in adult mouse brain to define the cancer ste...
Automated quantification of axonal growth
The aim of this project is to establish a standardised method for measuring cell and axonal growth from spinal cord slice cultures. To measure the area of axons outside the explant body, pictures of...
Effects of mixtures of endocrine disrupting compounds (EDC) on Wnt/beta-catenin signaling in developing...
Wnt/beta-catenin signaling is involved in proliferation and fate of cells thus playing fundamental roles during embryo development. The specific aims of this part of the EU project are to 1) develo...
Infiltration of T Cells in Thyroid Glands
The aim of the project is to estimate the degree of infiltration of T cells in thyroid glands of chicken in order to better understand auto-immunity and rare genetic disease. We have developed an im......
Assessing Bacterial Growth Kinetics and Morphology Using Time-lapse Microscopy Data
In vitro methods are often used to study the concentration-effect relationship for antimicrobial agents. Time-kill curve experiments have long been the standard methodology, with bacterial counts f...
Quantification of lipid droplets in human pre-adipocyte
Adipocytes store lipids, predominantly triglycerides (TGs), in lipid droplets (LDs). Upon energy shortage, TGs are hydrolyzed into non-esterified fatty acids and glycerol in an enzymatic process term...
Amyotrophic lateral sclerosis
Amyotrophic lateral sclerosis is a neurodegenerative disease characterized by the loss of motor neurons in the cortex brain stem and spinal chord. The incidence is 1 in 50 000 combining US and EU po...
Kidney Morphology and Topology of the Glomerular Filtration Barrier
Our collaborators have developed a super-resolution immunofluorescence microscopy protocol for the study of the filtration barrier in the kidney. The aim of the project is to quantitatively evaluate...
Analysis of Keratin Aggregates
Epidermolytic hyperkeratosis (EH) is a rare genetic skin disorder caused by mutation of keratin 1 or 10, and characterized by blistering in the epidermis and hyperkeratosis. The skin may blister eas...
Rat Spinal Cord
Our collaborators newly established an way of staining the activity in the endogenous opioid system in the rat spinal cord, and the aim of the project is to quantify the ammount and localization of ......
Cell Distribution and Protein Expression in the Ectocervix
The female genital mucosa presents a comprehensive natural immune defense against HIV infection, although during exposure to a high dose of virus this is not enough to protect the individual against...
Ubiquitin Screen
Ubiquitin is a small protein that is found in almost all cellular tissues in humans and other eukaryotic organisms, which helps to regulate the processes of other proteins in the body. Cultured cell...
Cell Time-Lapse Analysis
he aim of the project is to interpret differences in migration-proliferation of cells with different treatments and express those in a quantitative manner. We used a 'scratch assay' approach, or 'wo...
Vascular Networks
In this project we analyze vascular networks in the mouse brain, retina networks and cell junction activations. We have several applications where we skeletonize the networks and extract branch poin...
Segmentation of Neurons
The goal of this project is to analyze neurons grown from stem cells in vitro. The aim is to assess the percentage of neurons (using B-tubulin) and certain neuron subtypes (GABA) by immunofluorescen...
Quantification of Zebrafish Lipid Droplets
The aim of this project is to identify novel targets for the therapeutic intervention of coronary artery disease. This is done by following-up results from genome-wide association studies in epidemi...