Cell Time-Lapse Analysis

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Project Description

he aim of the project is to interpret differences in migration-proliferation of cells with different treatments and express those in a quantitative manner. We used a ‘scratch assay’ approach, or ‘wound healing assay’ as it sometimes is called, where cells are grown in wells, and then the surface is ‘scratched’ and loose cells are washed away. Then the wells are imaged, possibly followed by adding a drug substance, and imaging the wells again at a suitable time interval. The area filled at time point T is a measure of the migration speed.

Tags: Microscopy, cell biology