Illustration (click to hide): Assessing Bacterial Growth Kinetics and Morphology Using Time-lapse Microscopy Data

Project Description

In vitro methods are often used to study the concentration-effect relationship for antimicrobial agents. Time-kill curve experiments have long been the standard methodology, with bacterial counts followed over time using viable count assessments on agar plates. This method is labor-intensive and recently digital time-lapse microscopy methods have become available which might allow a more rapid assessment of antibiotic activity. Additionally, these methods could add information related to drug-induced morphological changes. The aim of this project is to integrate information obtained from time-lapse microscopy in the characterization of antibiotic effect on bacterial growth and morphology.


Tags: Microscopy, cell biology

Project Information

  • BIIF Principal Investigators

    • Petter Ranefall
    • Carolina Wählby

    External Authors

    Elisabet Nielsen - Dept. of Pharm. Biosci., UU, Chenyan Zhao, Pikkei Yuen, Pernilla Lagerbäck, Margreke Brill, Thomas Tängd&etilde;n, Otto Cars - Dept. of Med. Sci., UU
  • Date

    2016-06-03 🠚 2018-12-04