Imaging protein synthesis in primary cortical neuronal culture using Click-iT® Plus OPP Protein Synthesis Assay Kits

Illustration (click to hide): Imaging protein synthesis in primary cortical neuronal culture using Click-iT® Plus OPP Protein Synthesis Assay Kits

Project Description

The goal of this project is to analyze protein translation rate in mouse primary cortical neurons and astrocytes. The aim is to assess the protein synthesis by using the OPP Kit in primary cortical cultures of wild type and SLC38A10-/- Knockout mice. To understand role of SLC38A10 in protein regulation in neurons and astrocytes. We are using Cell Profiler to measure fluorescence intensity.


Tags: Microscopy, cell biology

Project Information

  • BIIF Principal Investigators

    • Petter Ranefall
    • Anna Klemm

    External Authors

    Rekha Tripathi, Pharmaceutical Biosciences, Uppsala University

  • Date

    2017-12-01 🠚 2019-11-15