Assessing Bacterial Growth Kinetics and Morphology Using Time-lapse Microscopy Data

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Project Description

In vitro methods are often used to study the concentration-effect relationship for antimicrobial agents. Time-kill curve experiments have long been the standard methodology, with bacterial counts followed over time using viable count assessments on agar plates. This method is labor-intensive and recently digital time-lapse microscopy methods have become available which might allow a more rapid assessment of antibiotic activity. Additionally, these methods could add information related to drug-induced morphological changes. The aim of this project is to integrate information obtained from time-lapse microscopy in the characterization of antibiotic effect on bacterial growth and morphology.

Tags: Microscopy, cell biology