A 3D human motor neuron disease platform for high throughput drug screening

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Project Description

The project developed a scalable 3D screening platform for analysing human motor neuron disease (MND) models using high-content microscopy. It segments neuron soma and neurite networks in 3D culture systems using hybrid models combining Cellpose and volumetric U-Nets. The workflow quantifies neurite outgrowth, soma size, and disease markers across drug-treated samples. Outputs support phenotype-based screening and therapeutic profiling in patient-derived iPSC models.